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Immunoblot shows alterations in protein expression and posttranslational modifications in response to hydroxyurea and entinostat. (A–D) Mouse PDAC cell lines (S821, 8248, 8296, S411) were treated with 1 µM entinostat (+; MS-275), 5 µM entinostat (++; MS-275) and/or 1 mM hydroxyurea (+; HU) for 24 h. Immunoblot was done for the indicated proteins and HSP90 as loading control; n = 2.
