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. 2021 Oct 1;10(10):2618. doi: 10.3390/cells10102618

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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LCA failed to activate the NLRP3 inflammasome in Nlrp3^−/− KC provoking no proinflammatory phenotype. Stimulation of Nlrp3^−/− KC with LCA (+Nigericin) did not activate the NLRP3 inflammasome with no increase of mRNA expression of Il-1b (B) and no change in neither intra- nor extracellular IL-1β protein levels (A,C). Percentage of pyroptotic, Caspase-1/PI double positive cells was the same after exposure to LCA + Nigericin in Nlrp3^−/− KC compared to basal levels (E). Still, LCA provoked formation of ROS in Nlrp3^−/− KC as comparison of DCF fluorescence showed (D). However, KC from Nlrp3^−/− mice showed no increase of proinflammatory mRNA markers (Tnf, iNos, Ccr8, Ccl22, ActivinA) after LCA stimulation (G) and percentage of Galectin 3-positive cells remained at a basal level (F). * p < 0.05; n.d.: not detected; ns: not significant.