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. 2021 Oct 14;13(20):5164. doi: 10.3390/cancers13205164

Figure 3.

Figure 3

(a) Experimental protocol for the preparation of blood samples by intracardiac puncture in nude mice (n = 5 animals per point) at the indicated times (4, 24, 48 h and 7 days, reported as “single dose”) after a single intraperitoneal administration of HoThyRu (4.5 mg/kg) or HoThyRu/DOTAP (15 mg/kg), or after weekly administrations of HoThyRu (4.5 mg/kg, i.p.) and HoThyRu/DOTAP (15 mg/kg, i.p.) once a week for 4 weeks (28 d), reported as “4 doses”. (b) Evaluation of ruthenium plasmatic levels over time throughout the in vivo study by ICP-MS analysis, as described in the experimental section. Results are plotted in bar graph as mg/L of total ruthenium in mice plasma samples (white bars refer to plasma levels at the indicated times after HoThyRu/DOTAP treatment; ribbed bars refer to plasma levels after HoThyRu treatment). *** p ≤ 0.005 vs. the HoThyRu-treated animal group.