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. 2021 Oct 7;10(10):2683. doi: 10.3390/cells10102683

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CCI induced neuronal damage in COs. (A) To analyze neuronal damage after CCI, mouse brain and COs slides were immunostained with NSE antibody. Images were analyzed using corrected total cellular fluorescence. (B) Significant increase in NSE staining in mouse brain after CCI compared to control. (C) Similarly, CCI in COs significantly increased NSE accumulation in cells compared to sham control, indicating neuronal damage. Statistical analysis performed with Student’s t-test, * p ≤ 0.05.