Figure 3.

Cell area and position of nuclei in different conditions. (A) Measurement of total cell area in different conditions. Each point corresponds to the average area of one cell over 5 time points. (B) Location of nucleus with respect to normalized cell length. Each point corresponds to the average of the nucleus positions of one cell over 5 time points. Graphs represent mean ± SEM of 12 cells (in static) and 13 cells (in motile) from 1–4 independent experiments per condition. For each cell, the measurements at 5 different time points (0, 30, 60, 90, and 120 min) were averaged. For statistics, a two-tailed Student’s t-test with unequal variance was used (** p < 0.01).