TABLE 2.
Plasmids used in this study
| Plasmid | Relevant characteristics | Reference |
|---|---|---|
| pSB1C3 mRFP1 | Cloning vector of this study. pUC19-derived pMB1 ori, chloramphenicol resistance, with mRFP1 (FPbase identifier [ID] 5YCFA) coding sequence |
iGEM part BBa_E1010 |
| pSB1C3 mTagBFP | Cloning vector. pUC19-derived pMB1 ori, chloramphenicol resistance, with mTagBFP (FPbase ID BFJKS) coding sequence |
iGEM part BBa_K592100 |
| pSB1C3 PihfBA-mTagBFP | pSB1C3 plasmid containing mTagBFP under the control of ihfB promoter | This study |
| pSB1C3 PxylA-mRFP1 | pSB1C3 plasmid containing mRFP1 under the control of xylA promoter | This study |
| pSB1C3 PxylA-mut-mRFP1 | pSB1C3 plasmid containing mRFP1 under the control of a mutated xylA
promoter, mutations were introduced in xylR binding sites |
This study |
| pSB1C3 PxylA-mRFP1 PihfB-mTagBFP | pSB1C3 plasmid containing mTagBFP under the control of ihfB promoter and mRFP1 under the control of xylA promoter |
This study |
| pSB1C3 PxylA | pSB1C3 plasmid containing xylA promoter | This study |
| pMET219 | Plasmid pBR322 with tetR but ampR replaced by cmR. Negative control of the plasmid pMET219 |
Gift from Sébastien Nouaille |
| pMET219 PcysG-xylR | Plasmid pMET219 with tetR replaced by xylR under control of cysG promoter | This study |