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. Author manuscript; available in PMC: 2021 Oct 22.
Published in final edited form as: Cell Rep. 2021 Aug 31;36(9):109597. doi: 10.1016/j.celrep.2021.109597

Figure 2. The pgPEN CRISPR library enables GI mapping of 1,030 human paralog pairs.

Figure 2.

(A) Schematic of pgPEN screening approach for paralog GI mapping.

(B) Violin plots of target-level CRISPR scores for negative control (double non-targeting control), positive control (single KO pgRNAs targeting known essential genes), all other single KO pgRNAs, and DKO pgRNAs in the PC9 screen. The double-targeting pgRNA group had significantly lower CRISPR scores than did the single-targeting pgRNA group (p < 2.20e–16 by one-tailed K-S test).

(C) Density plot of target-level CRISPR scores for DKO pgRNAs grouped by whether zero, one, or both targeted genes are expressed (TPM ≥ 2) in PC9 cells. Dashed lines indicate the median CRISPR score for each group. pgRNAs targeting expressed genes had significantly lower CRISPR scores than those targeting two unexpressed genes for both the 2/2 genes expressed (p < 2.20e–16 by one-tailed K-S test) and 1/2 genes expressed (p = 4.03e–03 by one-tailed K-S test) groups.

See also Figures S1 and S2 and Tables S1, S2, and S3.