Figure 9.

The influence of histone citrullination on the progress of histone degradation by C. albicans aspartic proteases. Unmodified (−) and deiminated histones (+) were treated with selected, recombinant aspartic proteinases (Saps, 6 nM) at a 1:1000 enzyme:substrate molar ratio (pH 5, 37 °C, 1 h). The reaction was stopped with pepstatin A (10 µM). The degradation profile was analyzed using SDS-PAGE in the Schägger von Jagov system with silver staining after glutaraldehyde fixation for the protein band visualization, and the representative result of three experiments is presented.