Figure 3.

SHQA prevents cAMP production and PKA activation in B16F10 cells. (A) After pre-treatment of SHQA for 1 h, cells were treated with α-MSH (1.0 μM) for 30 min with or without SHQA. Intracellular cAMP concentration was measured by a cAMP ELISA kit. (B) Cell lysates were immunoprecipitated with anti-PKA-RIIβ antibody and PKA-Cα co-precipitation was tested by western blotting. The band images were measured by densitometry and shown as bar graphs. Data are expressed as the mean ± standard deviation (SD) (n = 3). Different superscripts represent statistical significance at p < 0.05.