Figure 2.

Characterization of the SZT2 interactome. (A) Principal component analysis (PCA) of the abundancies of all proteins identified in the interactome of SZT2 under starvation (light violet) or stimulation (violet) and of the GFP control (black). (B) Zoom in of volcano plot of all identified proteins interacting with SZT2 or the GFP control (see Figure S2A for whole volcano plot). The gray color scale depicts the number of proteins that have the same abundance and p-value. Proteins significantly binding to SZT2 have a p-value below 0.05 and an abundance ratio of 5 over the GFP control (within dotted lines). Representative components of KICSTOR and GATOR1 complexes are depicted in blue labeled with their protein names. (C) Summary of Top9 processes (KEGG pathway and WikiPathway) associated with proteins binding with high affinity to SZT2. Relevant processes were selected based on their −log10 (p-value). Maps of (D) autophagy, (E) cilia-associated terms, (F) neurological disorder-associated terms, with the depiction of proteins, which have a significantly higher affinity to bind to SZT2 than to the GFP control. The node size correlates with the number of mapped proteins per term and the node color with the significance of this term.