Table 2.

Agreement of PD-L1 expression in paired histological and cytological non-small cell lung cancer specimens with different cytological sample types specified.

PD-L1		All Cases	Pleural Effusions	Bronchial Brushes	Bronchoalveolar Lavages
Cutoff ≥1% positive cells (95% CI)	Kappa (κ)	0.70 (0.56–0.83)	0.48 (0.08–0.87)	0.55 (0.21–0.90)	0.81 (0.66–0.95)
	OPA	85 (77–91)	74 (51–89)	76 (52–91)	91 (81–96)
	PPA	89 (77–95)	70 (39–90)	100 (56–100)	92 (78–98)
	NPA	81 (67–90)	78 (44–95)	64 (35–85)	89 (71–97)
	McNemar	0.61	1.00	0.134	0.683
Cutoff ≥5% positive cells (95% CI)	Kappa (κ)	0.53 (0.37–0.70)	0.42 (−0.01–0.84)	0.65 (0.32–0.99)	0.53 (0.33–0.74)
	OPA	77 (68–84)	74 (51–89)	82 (58–95)	77 (65–85)
	PPA	73 (59–84)	57 (25–84)	100 (56–100)	72 (54–85)
	NPA	80 (67–89)	83 (54–97)	73 (43–91)	81 (64–91)
	McNemar	1.00	1.00	0.248	0.606
Cutoff ≥10% positive cells (95% CI)	Kappa (κ)	0.51 (0.34–0.69)	0.07 (−0.39–0.52)	0.64 (0.29–0.99)	0.56 (0.35–0.76)
	OPA	77 (68–84)	68 (46–85)	82 (58–95)	78 (66–87)
	PPA	71 (55–83)	20 (2–64)	100 (51–100)	75 (56–88)
	NPA	81 (69–89)	86 (59–97)	75 (46–92)	81 (65–91)
	McNemar	1.00	0.683	0.248	0.789
Cutoff ≥50% positive cells (95% CI)	Kappa (κ)	0.53 (0.34–0.72)	−0.16 (−0.33–0.00)	0.85 (0.57–1.00)	0.58 (0.36–0.80)
	OPA	82 (73–89)	68 (46–85)	94 (71–100)	83 (72–90)
	PPA	63 (44–79)	00 (00–55)	100 (45–100)	68 (46–85)
	NPA	89 (80–95)	87 (61–98)	92 (65–100)	89 (76–96)
	McNemar	0.81	0.683	1.00	1.00

Kappa (κ) is presented as range −1–1. OPA, PPA, and NPA are presented as percent with histology as the non-reference standard. McNemar analyses are presented as p-values; CI: confidence interval; Kappa (κ): Cohen’s kappa coefficient; NPA: Negative Percentage Agreement; OPA: Overall Percentage Agreement; PPA: Positive Percentage Agreement.