Table 3.
Association of PD-L1 concordance for paired histological and cytological non-small cell lung cancer specimens with tumour type, specimen type, and cell content.
| PD-L1 | All Cases | Pleural Effusions | Bronchial Brushes | Bronchoalveolar Lavages | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Concordant | Discordant | p Values | Concordant | Discordant | p Values | Concordant | Discordant | p Values | Concordant | Discordant | p Values | ||
| Cut-off ≥1% positive cells | All cases, n (%) | 85/100 (85) |
15/100 (15) |
14/19 (74) |
5/19 (26) |
13/17 (77) |
4/17 (24) |
58/64 (91) |
6/64 (9) |
||||
| Histological subtype, n (%) | 0.77 | n/a | 0.60 | 0.40 | |||||||||
| Adenocarcinoma | 55/66 (83) |
11/66 (17) |
14/19 (74) |
5/19 (26) |
6/7 (86) |
1/7 (14) |
35/40 (88) |
5/40 (13) |
|||||
| Squamous cell carcinoma | 30/34 (88) |
4/34 (12) |
0/0 (00) |
0/0 (00) |
7/10 (70) |
3/10 (30) |
23/24 (96) |
1/24 (4) |
|||||
| Type of biopsy, n (%) | 0.17 | 0.63 | 1.00 | 0.51 | |||||||||
| Bronchial biopsy | 70/80 (88) |
10/80 (13) |
8/10 (80) |
2/10 (20) |
10/13 (77) |
3/13 (23) |
52/57 (91) |
5/57 (9) |
|||||
| Transthoracic core biopsy | 15/20 (75) |
5/20 (25) |
6/9 (67) |
3/9 (33) |
3/4 (75) |
1/4 (25) |
6/7 (86) |
1/7 (14) |
|||||
| Specimen cell amount “Cell blocks”, n (%) |
0.34 | 0.23 | 0.28 | 0.52 | |||||||||
| 100–300 | 37/47 (79) |
10/47 (21) |
1/3 (33) |
2/3 (67) |
5/8 (63) |
3/8 (38) |
31/36 (86) |
5/36 (14) |
|||||
| >300–500 | 20/23 (87) |
3/23 (13) |
4/5 (80) |
1/5 (20) |
1/2 (50) |
1/2 (50) |
15/16 (94) |
1/16 (6) |
|||||
| >500–1000 | 8/9 (89) |
1/9 (11) |
1/2 (50) |
1/2 (50) |
5/5 (100) |
0/5 (00) |
2/2 (100) |
0/2 (00) |
|||||
| >1000 | 20/21 (95) |
1/21 (5) |
8/9 (89) |
1/9 (11) |
2/2 (100) |
0/2 (00) |
10/10 (100) |
0/10 (00) |
|||||
| Specimen cell amount “Biopsies”, n (%) |
0.46 | 0.05 | 0.21 | 0.97 | |||||||||
| 100–300 | 37/44 (84) |
7/44 (16) |
9/10 (90) |
1/10 (10) |
3/6 (50) |
3/6 (50) |
25/28 (89) |
3/28 (11) |
|||||
| >300–500 | 16/18 (89) |
2/18 (11) |
1/2 (50) |
1/2 (50) |
4/4 (100) |
0/4 (00) |
11/12 (92) |
1/12 (8) |
|||||
| >500–1000 | 21/23 (91) |
2/23 (9) |
4/5 (80) |
1/5 (20) |
3/3 (100) |
0/3 (00) |
14/15 (93) |
1/15 (7) |
|||||
| >1000 | 11/15 (73) |
4/15 (27) |
0/2 (00) |
2/2 (100) |
3/4 (75) |
1/4 (25) |
8/9 (89) |
1/9 (11) |
|||||
| Cut-off ≥5% positive cells | All cases, n (%) | 77/100 (77) |
23/100 (23) |
14/19 (74) |
5/19 (26) |
14/17 (82) |
3/17 (18) |
49/64 (77) |
15/64 (23) |
||||
| Histological subtype, n (%) | 0.21 | n/a | 1.00 | 0.14 | |||||||||
| Adenocarcinoma | 48/66 (73) |
18/66 (27) |
14/19 (74) |
5/15 (26) |
6/7 (86) |
1/7 (14) |
28/40 (70) |
12/40 (30) |
|||||
| Squamous cell carcinoma | 29/34 (85) |
5/34 (15) |
0/0 (00) |
0/0 (00) |
8/10 (80) |
2/10 (20) |
21/24 (88) |
3/24 (13) |
|||||
| Type of biopsy, n (%) | 0.77 | 0.63 | 1.00 | 1.00 | |||||||||
| Bronchial biopsy | 62/80 (78) |
18/80 (23) |
8/10 (80) |
2/10 (20) |
11/13 (85) |
2/13 (15) |
43/57 (75) |
14/57 (25) |
|||||
| Transthoracic core biopsy | 15/20 (75) |
5/20 (25) |
6/9 (67) |
3/9 (33) |
3/4 (75) |
1/4 (25) |
6/7 (86) |
1/7 (14) |
|||||
| Specimen cell amount “Cell blocks”, n (%) |
0.35 | 0.48 | 0.36 | 0.43 | |||||||||
| 100–300 | 34/47 (72) |
13/47 (28) |
3/3 (100) |
0/3 (00) |
6/8 (75) |
2/8 (25) |
25/36 (69) |
11/36 (31) |
|||||
| >300–500 | 18/23 (78) |
5/23 (22) |
3/5 (60) |
2/5 (40) |
1/2 (50) |
1/2 (50) |
14/16 (88) |
2/16 (13) |
|||||
| >500–1000 | 9/9 (100) |
0/9 (00) |
2/2 (100) |
0/2 (00) |
5/5 (100) |
0/5 (00) |
2/2 (100) |
0/2 (00) |
|||||
| >1000 | 16/21 (76) |
5/21 (24) |
6/9 (67) |
3/9 (33) |
2/2 (100) |
0/2 (00) |
8/10 (80) |
2/10 (20) |
|||||
| Specimen cell amount “Biopsies”, n (%) |
0.32 | 0.69 | 0.45 | 0.045 | |||||||||
| 100–300 | 35/44 (80) |
9/44 (21) |
8/10 (80) |
2/10 (20) |
4/6 (67) |
2/6 (33) |
23/28 (82) |
5/28 (18) |
|||||
| >300–500 | 11/18 (61) |
7/18 (39) |
1/2 (50) |
1/2 (50) |
4/4 (100) |
0/4 (00) |
6/12 (50) |
6/12 (50) |
|||||
| >500–1000 | 18/23 (78) |
5/23 (22) |
4/5 (80) |
1/5 (20) |
3/3 (100) |
0/3 (00) |
11/15 (73) |
4/15 (27) |
|||||
| >1000 | 13/15 (87) |
2/15 (13) |
1/2 (50) |
1/2 (50) |
3/4 (75) |
1/4 (25) |
9/9 (100) |
0/9 (00) |
|||||
| Cut-off ≥10% positive cells | All cases, n (%) | 77/100 (77) |
23/100 (23) |
13/19 (68) |
6/19 (32) |
14/17 (82) |
3/17 (18) |
50/64 (78) |
14/64 (22) |
||||
| Histological subtype, n (%) | 0.21 | n/a | 1.00 | 0.22 | |||||||||
| Adenocarcinoma | 48/66 (73) |
18/66 (27) |
13/19 (68) |
6/19 (32) |
6/7 (86) |
1/7 (14) |
29/40 (73) |
11/40 (28) |
|||||
| Squamous cell carcinoma | 29/34 (85) |
5/34 (15) |
0/0 (00) |
0/0 (00) |
8/10 (80) |
2/10 (20) |
21/24 (88) |
3/24 (13) |
|||||
| Type of biopsy, n (%) | 0.77 | 1.00 | 1.00 | 1.00 | |||||||||
| Bronchial biopsy | 62/80 (78) |
18/80 (23) |
7/10 (70) |
3/10 (30) |
11/13 (85) |
2/13 (15) |
44/57 (77) |
13/57 (23) |
|||||
| Transthoracic core biopsy | 15/20 (75) |
5/20 (25) |
6/9 (67) |
3/9 (33) |
3/4 (75) |
1/4 (25) |
6/7 (86) |
1/7 (14) |
|||||
| Specimen cell amount “Cell blocks”, n (%) |
0.75 | 0.78 | 0.57 | 0.53 | |||||||||
| 100–300 | 35/47 (75) |
12/47 (26) |
2/3 (67) |
1/3 (33) |
7/8 (88) |
1/8 (13) |
26/36 (72) |
10/36 (28) |
|||||
| >300–500 | 17/23 (74) |
6/23 (26) |
3/5 (60) |
2/5 (40) |
1/2 (50) |
1/2 (50) |
13/16 (81) |
3/16 (19) |
|||||
| >500–1000 | 8/9 (89) |
1/9 (11) |
2/2 (100) |
0/2 (00) |
4/5 (80) |
1/5 (20) |
2/2 (100) |
0/2 (00) |
|||||
| >1000 | 17/21 (81) |
4/21 (19) |
6/9 (67) |
3/9 (33) |
2/2 (100) |
0/2 (00) |
9/10 (90) |
1/10 (10) |
|||||
| Specimen cell amount “Biopsies”, n (%) |
0.18 | 0.70 | 0.82 | 0.15 | |||||||||
| 100–300 | 34/44 (77) |
10/44 (23) |
7/10 (70) |
3/10 (30) |
5/6 (83) |
1/6 (17) |
22/28 (79) |
6/28 (21) |
|||||
| >300–500 | 11/18 (61) |
7/18 (39) |
1/2 (50) |
1/2 (50) |
3/4 (75) |
1/4 (25) |
7/12 (58) |
5/12 (42) |
|||||
| >500–1000 | 18/23 (78) |
5/23 (22) |
3/5 (60) |
2/5 (40) |
3/3 (100) |
0/3 (00) |
12/15 (80) |
3/15 (20) |
|||||
| >1000 | 14/15 (93) |
1/15 (7) |
2/2 (100) |
0/2 (00) |
3/4 (75) |
1/4 (25) |
9/9 (100) |
0/9 (00) |
|||||
| Cut-off ≥50% positive cells | All cases, n (%) | 82/100 (82) |
18/100 (18) |
13/19 (68) |
6/19 (32) |
16/17 (94) |
1/17 (6) |
53/64 (83) |
11/64 (17) |
||||
| Histological subtype, n (%) | 0.11 | n/a | 0.41 | 0.51 | |||||||||
| Adenocarcinoma | 51/66 (77) |
15/66 (23) |
13/19 (68) |
6/19 (32) |
6/7 (86) |
1/7 (14) |
32/40 (80) |
8/40 (20) |
|||||
| Squamous cell carcinoma | 31/34 (91) |
3/34 (9) |
0/0 (00) |
0/0 (00) |
10/10 (100) |
0/10 (00) |
21/24 (88) |
3/24 (13) |
|||||
| Type of biopsy, n (%) | 0.75 | 1.00 | 1.00 | 1.00 | |||||||||
| Bronchial biopsy | 66/80 (83) |
14/80 (18) |
7/10 (70) |
3/10 (30) |
12/13 (92) |
1/13 (8) |
47/57 (83) |
10/57 (18) |
|||||
| Transthoracic core biopsy | 16/20 (80) |
4/20 (20) |
6/9 (67) |
3/9 (33) |
4/4 (100) |
0/4 (00) |
6/7 (86) |
1/7 (14) |
|||||
| Specimen cell amount “Cell blocks”, n (%) |
0.31 | 0.78 | 0.47 | 0.35 | |||||||||
| 100–300 | 41/47 (87) |
6/47 (13) |
2/3 (67) |
1/3 (33) |
8/8 (100) |
0/8 (00) |
31/36 (86) |
5/36 (14) |
|||||
| >300–500 | 16/23 (70) |
7/23 (30) |
3/5 (60) |
2/5 (40) |
2/2 (100) |
0/2 (00) |
11/16 (69) |
5/16 (31) |
|||||
| >500–1000 | 8/9 (89) |
1/9 (11) |
2/2 (100) |
0/2 (00) |
4/5 (80) |
1/5 (20) |
2/2 (100) |
0/2 (00) |
|||||
| >1000 | 17/21 (81) |
4/21 (19) |
6/9 (67) |
3/9 (33) |
2/2 (100) |
0/2 (00) |
9/10 (90) |
1/10 (10) |
|||||
| Specimen cell amount “Biopsies”, n (%) |
0.017 | 0.70 | 0.33 | 0.016 | |||||||||
| 100–300 | 35/44 (80) |
9/44 (21) |
7/10 (70) |
3/10 (30) |
6/6 (100) |
0/6 (00) |
22/28 (79) |
6/28 (21) |
|||||
| >300–500 | 11/18 (61) |
7/18 (39) |
1/2 (50) |
1/2 (50) |
3/4 (75) |
1/4 (25) |
7/12 (58) |
5/12 (42) |
|||||
| >500–1000 | 21/23 (91) |
2/23 (9) |
3/5 (60) |
2/5 (40) |
3/3 (100) |
0/3 (00) |
15/15 (100) |
0/15 (00) |
|||||
| >1000 | 15/15 (100) |
0/15 (00) |
2/2 (100) |
0/2 (00) |
4/4 (100) |
0/4 (00) |
9/9 (100) |
0/9 (00) |
|||||