Item No	Recommendation	Please Insert Check Where Included or N/A Where Not Applicable
Title and abstract	1	(a) Indicate the study’s design with a commonly used term in the title or the abstract	check
		(b) Provide, in the abstract, an informative and balanced summary of what was performed and what was found	check
Introduction
Background/rationale	2	Explain the scientific background and rationale for the investigation being reported	check
Objectives	3	State specific objectives, including any pre specified hypotheses	check
Methods
Study design	4	Present key elements of study design early in the paper	check
Setting	5	Describe the setting, locations and relevant dates, including periods of recruitment, treatment, follow up and data collection	check
Participants	6	(a) Cohort study—Provide the eligibility criteria and the sources and methods of selection of participants. Describe methods of follow up Case-control study—Provide the eligibility criteria and the sources and methods of case ascertainment and control selection. Provide the rationale for the choice of cases and controls Cross-sectional study—Provide the eligibility criteria and the sources and methods of selection of participants	check
		(b) Cohort study—For matched studies, provide matching criteria and number of treated and untreated Case-control study—For matched studies, provide matching criteria and the number of controls per case
Variables	7	Clearly define all outcomes, exposures, predictors, potential confounders and effect modifiers. Provide diagnostic criteria, if applicable	check
Data sources/measurement	8 *	For each variable of interest, provide sources of data and details of methods of assessment (measurement) Describe comparability of assessment methods if there is more than one group	check
Bias	9	Describe any efforts to address potential sources of bias	check
Study size	10	Explain how the study size was arrived at	check
Quantitative variables	11	Explain how quantitative variables were handled in the analyses. If applicable, describe which groupings were chosen and why	check
Statistical methods	12	(a) Describe all statistical methods, including those used to control for confounding factors	check
/		(b) Describe any methods used to examine subgroups and interactions	/
/		(c) Explain how missing data were addressed	check
/		(d) If applicable, explain how loss to follow up was addressed	check
/		(e) Describe any sensitivity analyses	/
Results
Participants	13 *	(a) Report numbers of individuals at each stage of study—e.g., numbers potentially eligible, examined for eligibility, confirmed eligible, included in the study, completing follow up and analyzed	check
/		(b) Give reasons for nonparticipation at each stage	check
Descriptive data	14 *	(a) Give characteristics of study participants (e.g., demographic, clinical, social) and information on other treatments and potential confounders	check
/		(b) Indicate number of participants with missing data for each variable of interest	check
/		(c) Cohort study—Summarize follow-up time (e.g., average and total amount)	check
Outcome data	15 *	Report numbers of outcome events or summary measures over time	N/A
Main results	16	(a) Provide unadjusted estimates and, if applicable, confounder-adjusted estimates and their precision (e.g., 95% confidence interval). Make clear which confounders were adjusted for and why they were included	/
/		(b) Report category boundaries when continuous variables were categorized	N/A
/		(c) If relevant, consider translating estimates of relative risk into absolute risk for a meaningful time period	N/A
Other analyses	17	Report other analyses performed—e.g., analyses of subgroups and interactions and sensitivity analyses	N/A
Discussion
Key results	18	Summarise key results with reference to study objectives	check
Limitations	19	Discuss limitations of the study, taking into account sources of potential bias or imprecision. Discuss both direction and magnitude of any potential bias	check
Interpretation	20	Provide a cautious overall interpretation of results considering objectives, limitations, multiplicity of analyses, results from similar studies and other relevant evidence	check
Generalisability	21	Discuss the generalisability (external validity) of the study results	check
Other information
Funding	22	Provide the source of funding and the role of the funders for the present study and, if applicable, for the original study on which the present article is based	check
* Provide information separately for cases and controls. Note: An Explanation and Elaboration article discusses each checklist item and provides methodological background and published examples of transparent reporting. Information on the STROBE Initiative is available at http://www.strobe-statement.org, access date 15 January 2021.