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. 2021 Oct 16;10(10):2775. doi: 10.3390/cells10102775

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Deletion of PaIap prevents proteolytic turnover of PaCRD1. (A): Western blot analysis of isolated mitochondria of 7-day-old wild type (WT) and ΔPaIap. Coomassie staining was used as loading control. (B): Quantification of PaCRD1 in (A). PaCRD1 levels were determined and related to wild type (set to 1). Data represent mean ± SD (n = 5). ** p < 0.01. (C): Transcript analysis of 5-day-old wild type and ΔPaIap grown on M2. PaCrd1 transcript level was analyzed by qPCR and normalized to PaPorin expression level. Data represent mean ± SD (wild type: n = 6, ΔPaIap: n = 3).