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. 2021 Oct 16;10(10):2775. doi: 10.3390/cells10102775

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Loss of PaIAP enhances mitochondrial turnover. (A): Southern blot analysis of HindIII-digested DNA of the generated mitophagy reporter strain ΔPaIap/PaSod3^H26L::mCherry. (B): Western blot analysis of isolated protein extracts of 6-day-old PaSod3^H26L::mCherry (here WT) and ΔPaIap/PaSod3^H26L::mCherry (here ΔPaIap) grown in liquid CM medium. Coomassie staining was used as loading control. (C): Quantification of mitophagy rate in (B). Mitophagy rate was determined by ratio of “free mCHERRY” to total “free mCHERRY” + PaSOD3^H26L::mCHERRY (fusion protein). Data represent mean ± SD (PaSod3^H26L::mCherry n = 9; ΔPaIap/PaSod3^H26L::mCherry n = 10). *** p < 0.001.