TABLE 3.

SNPs mapped in the mutants with low susceptibility to fosfomycin

Mutant	Position of the reference sequence	SNP	Locus	Gene	Amino acid changea	Product	Old locus tag	Provean scoreb	SNP domain
FOS1	1829461	A:T>G:C	SMD_RS08765	eno	p.D398G	Enolase	SMD_1655	−6.77	C-terminal TIM barrel domain
FOS4	1411119	C:G>T:A	SMD_RS06650	gpmA	p.P212L	Phosphoglycerate mutase	SMD_1268	−9.88	Histidine phosphatase superfamily
FOS7	3798418	G:C>C:G	SMD_RS17680	gapA	p.D296G	Glyceraldehyde-3- phosphate dehydrogenase	SMD_3406	−3.37	NAD binding domain
FOS8	3793349	G:C>A:T	SMD_RS17665	pgk	p.Q50ST	Phosphoglycerate kinase	SMD_3403		N-terminal domain, containing the substrate binding site

^aST, stop mutation.

^bProvean deleterious score threshold of the changes is −2.5. Any SNP is located in a catalytic site of the corresponding enzyme.