Figure 1.

Severe acute respiratory syndrome coronavirus 2 protein constructs. (A) Schematic representations of the primary structure of full-length sequence of the spike protein (S) and schematic representation of constructs, namely pcDNA3.1/S1 and pcDNA3.1/N, with a 6XHis tag at the C-terminal and spike signal sequence at the N-terminal, which is flanked by XhoI and XbaI. (B) Enzymatic digestion of the synthesized recombinant plasmids, which were analyzed on a 1.2% (w/v) agarose gel. Lane 1, 5000 bp DNA size marker; lane 2, undigested plasmids; lane 3, enzymatic digestion of pcDNA3.1/S1 and pcDNA3.1/N with Xho and KpnI.