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. 2021 Sep 30;11(10):1808. doi: 10.3390/diagnostics11101808

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Enzyme-linked immunosorbent assay to determine recognition of the S1 domain of spike protein, nucleocapsid, and RBD by serum samples from patients with confirmed COVID-19. Ten serum samples from patients with serologically confirmed COVID-19 and 8 control serum samples were evaluated at a dilution of 1:100, and a horseradish peroxidase-conjugated rabbit anti-human IgG was used as the secondary antibody. Bars represent the mean ± SD., *** p < 0.001. p ≤ 0.05 was considered significant. Prism v.6 software was used for statistical analyses (GraphPad Inc., La Jolla, CA, USA).