Table 1.
ACE inhibitory activity (%) of CPI hydrolyzed with Flavourzyme or Alcalase at different times. Maximal inhibitory activity (IC50) of CPHs obtained using Alcalase on the ACE enzyme.
| Hydrolysis Time (min) | CPH Flavourzyme | CPH Alcalase | |
|---|---|---|---|
| ACE Inhibition (%) | ACE Inhibition (%) | IC50 (µg/mL) | |
| 0 | 15.92 ± 0.27 | 12.33 ± 0.54 | |
| 15 | 42.19 ± 0.53 | 82.85 ± 0.42 | 78.84 ± 1.21 |
| 30 | 36.17 ± 0.83 | 80.13 ± 0.40 | 74.63 ± 0.53 |
| 45 | 40.50 ± 0.40 | 82.16 ± 0.25 | 67.50 ± 0.44 |
| 60 | 36.66 ± 0.62 | 82.84 ± 0.13 | 68.06 ± 0.67 |
| 75 | 28.79 ± 0.74 | ||
| 90 | 34.33 ± 0.84 | ||
| 105 | 30.36 ± 0.61 | ||
| 120 | 31.83 ± 2.04 | ||