Figure 4.

Administration of L. lactis secreting SP-1 influences colitis. (a) C57BL/6 mice were provided with water or 1.5% DSS-containing water for 7 days (N = 8 in each group). Mice were orally administered with PBS, PBS-L. lactis-WT, PBS-L. lactis-SP-1, DSS alone, DSS-L. lactis-WT or DSS-L. lactis- SP-1 (5 × 10⁹ CFU L. lactis-WT or SP-1-expressing L. lactis were evaluated / day). (b) Body weight loss for each group. (c–e) Mice were euthanized (day 8), and (c) colon length, (d) disease activity index, and (e) colonic MPO activity were measured. (f) Histological scores were determined. Data are presented as mean ± SEM from a representative experiment (N = 8 biologically independent animals). Analyzed by Kruskal–Wallis followed by the multi-comparison Dunn’s test. * p < 0.05; ** p < 0.01 and *** p < 0.001.