FIG. 4.

Subcellular localization of CIZ. (A to F) 3Y1 cells were grown overnight on the uncoated coverslips. (G and H) After overnight culture, 3Y1 cells were treated with LMB (10 ng/ml) for 24 h. Immunofluorescence was performed with anti-CIZ (1:400) (A, C, and G) precleared by GST or with anti-CIZ precleared by GST-CIZ (E), together with antivinculin antibody (B, D, F, and H) as described previously (31). Panels A, B, G, and H show the sections at the height of focal adhesions. Panels C, D, E, and F show the sections at the height of the nucleus.