FIG. 6.

CIZ has DNA-binding consensus sequences. (A) Results of CASTing described in Materials and Methods. (B) Overexpressed CIZ binds to the CCTTTTTCAAAAAGA sequence of the human MMP-1 promoter. EMSA was performed with the nuclear extract of CIZ-FLAG (lanes 2 to 5)-transfected cells and with that of mock (lane 1)-transfected COS-7 cells. The horizontally lined arrowhead indicates the DNA-protein complex (lanes 2, 4, and 6). The supershifted band is indicated by the vertically lined arrowhead (lane 5). The background band caused by immunoglobulin was indicated by the open arrowhead (lanes 5 and 6). (C) Endogeneous CIZ is included in the DNA-protein complex (lane 7). The supershift band was observed in panel B (lane 10).