FIG. 7.

CIZ upregulates the expression of MMPs. (A) CIZ transactivates the artificial promoter consisting of four tandem repeats of CIZ-binding consensus sequences in the human MMP-1 promoter. (B to D) CIZ upregulates the transcription from the human MMP-1 promoter mediated by the CIZ-binding consensus sequence (B) from the MMP-3 promoter (C) and from the MMP-7 promoter (D). vec, vector. The effect of Cas expression on the transactivation by CIZ was checked in Cas^−/− fibroblasts (E). In these experiments, NIH 3T3 cells or Cas^−/− fibroblasts were transfected with each expression vector, together with the reporter construct and β-galactosidase expression vector. Values are the means of three experiments, with bars representing the standard deviations. (F and G) Overproduction of MMP-7 in HT1080 cells stably expressing CIZ. (F) Total lysates of m-1 (lane 1), m-2 (lane 2), C-1 (lane-3), and C-2 (lane-4) cells were immunoblotted (IB) with anti-CIZ. (G) The conditioned media of m-1 (lane 1), m-2 (lane 2), C-1 (lane 3), and C-2 (lane 4) cells were immunoblotted with anti-proMMP-7.