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. 2021 Sep 22;10(10):2242. doi: 10.3390/foods10102242

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Oxidative stress measurements in HepG2 cells treated with TEB. (A) Fluorescence images of hydrogen peroxide (green) and superoxide (red) in cells (200× magnification, white bar = 100µm); quantification of the (B) green and (C) red fluorescence ratio in cells. Cells were exposed to 20, 40, and 80 µM TEB for 12 and 24 h with or without pretreatment with 20 µM Lipofermata for 1 h (n = 3 wells/group). The data are represented as mean ± SEM. ** (p < 0.01) and *** (p < 0.001) show a significant difference, compared to the control (DMSO). # (p < 0.05) and ## (p < 0.01) show a significant difference, compared to the TEB-treated cells.