Table 4.
Validation parameters in HPLC flavonoid determination.
| Linear Equation |
R2 | Coefficient Linearity (%) | LOD (μg/mL) | LOQ (μg/mL) | Accuracy Injection (%RSD) | Repeatability (%RSD) | Reproducibility (%RSD) | |
|---|---|---|---|---|---|---|---|---|
| (+)-Catechin | 108.7x − 3.6 | 0.9937 | 99 | 0.01 | 0.02 | 4.0 | 6.4 | 9.3 |
| (−)-Epicatechin | 107.1x − 4.0 | 0.9848 | 99 | 0.02 | 0.03 | 0.6 | 4.7 | 10.6 |
| Isorhamnetin-3-O-glucoside | 159.4x + 0.59 | 0.9988 | 99 | 0.01 | 0.03 | 1.3 | 1.9 | 6.5 |
| Kaempferol-3-O-glucoside | 219.4x − 3.3 | 0.9808 | 100 | 0.007 | 0.01 | 1.8 | 3.3 | 6.6 |
| Isorhamnetin-3-O-rutinoside | 175.7x + 0.92 | 0.9961 | 99 | 0.01 | 0.02 | 0.3 | 2.0 | 10.9 |