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. 2021 Oct 13;10(10):2730. doi: 10.3390/cells10102730

Figure 2.

Figure 2

mTORC1-induced ER stress targets LAMP2 via ATF4-CHOP pathway under FFA treatment in hepatocytes. Hepa-1c1c7 cells were treated with PA alone, or in combination with rapamycin in the absence or presence of a potent ER stress inducer, tunicamycin (TM), at 5 µM for 24 h. (A) Immunoblot and quantification analysis of cellular protein levels of p-S6, S6, ATF4, CHOP, LAMP2, and LC3II. (B) Hepa-1c1c7 cells, with or without transfection of CHOP knockdown CRISPR plasmid, were treated with 100 µM PA for 24 h and immunoblotted for cellular p-S6, S6, ATF4, CHOP, and LAMP2. A one-way ANOVA test was performed to calculate the significance of the data (* p < 0.05).