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. 2021 Oct 13;10(10):2730. doi: 10.3390/cells10102730

Figure 4.

Figure 4

Inhibition of mTOR signaling by dietary rapamycin supplementation attenuates alcohol-induced lipid accumulation and liver injury in mice. WT mice were fed a control (PF) or alcohol liquid diet (AF) with or without rapamycin supplementation at 0.04 mg/mouse/day, three times a week, starting from the 6th week in an 8-week feeding experiment. (A) Analysis of hepatic FFA and TG contents (n = 4/group). (B) H&E staining of liver tissue sections (n = 4/group). Arrowheads: hepatocyte degeneration. Arrows: hepatic lipid droplets. Scale bars, 20 μm. (C) Analysis of serum ALT and AST levels (n = 4/group). (D) Immunohistochemistry staining of hepatic 4-HNE on liver tissue sections. Scale bars, 20 μm. (E) Hepatic Cxcl1 and Ly6g mRNA expression (n = 4/group). (F) Immunofluorescence staining of hepatic MPO positive cells (red) (n = 4/group). Scale bars, 20 μm. Data are shown as means ± SD. A one-way ANOVA test was performed to calculate the significance of the data (* p < 0.05).