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. 2021 Oct 9;10(10):2701. doi: 10.3390/cells10102701

Figure 5.

Figure 5

STIM1 induces cytoskeletal remodeling in NRCM. (A) Blot showing F- and G- actin in the STIM1 overexpressing NRCM. GSN knockdown cells there is accumulation of F-actin, in contrast to cells transfected with scrambled GSN shRNA. (B) Quantification analysis of F/G actin ratios. (C) NRCM transiently overexpressing STIM1-YFP (a) and co-stained with anti-GSN antibody (b) Merging the fluorescence signal reveals colocalization between STIM1 and GSN (c). (D) Line-scan profiles of fluorescence intensity for NRCM (form C, a and b). The green line indicates STIM1 spatial distribution, and the red line indicates GSN distribution. (E) Micrographs of NRCM transiently expressing STIM1-YFP (a) and stained with anti-tubulin antibody (b), merged channels (c). (F) Tubulin quantification by fluorescence intensity between NRCM and NRCM expressing STIM1-YFP. Data are from three independent experiments, * p < 0.05, *** p < 0.001.