FIG. 2.

(A) Expression of Vav Y3xF in transformed cells. Exponentially growing NIH 3T3 cell clones obtained from randomly picked foci from Vav and Vav Y3xF transfections were lysed, immunoprecipitated with anti-Vav antibodies, and subjected to immunoblot analysis with anti-Vav antibodies. The mobilities of Vav and the immunoglobulin G (IgG) heavy chain are indicated by arrows. The migration of coelectrophoresed molecular weight markers is indicated on the right. WT, wild type. (B) Growth kinetics of parental NIH 3T3 cells (diamonds) and NIH 3T3 cell clones transformed by the expression of wild-type Vav (triangles), Vav (Δ1-186) (squares), and Vav Y3xF (circles). Exponentially growing cultures were trypsinized and seeded in duplicate in six-well plates (30,000 cells/well), and cells were counted at the indicated times. Values at each time point represent the mean of duplicate determinations performed in parallel. The value at day 0 represents the number of cells present 1 day after seeding to eliminate differences in cell growth due to variable plating efficiencies for the different cell clones.