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15dPGJ2 decreases the mRNA levels of COX-2 and NOS-2. Cells were incubated with the indicated concentrations of PG and activated with LPS (200 ng/ml) and IFN-γ (10 U/ml). After 4 h of treatment, the cells were homogenized and the RNA was extracted and analyzed by Northern blotting using probes specific for the indicated genes. Results show the mean band intensity expressed as a percentage of that in the absence of PG and after normalization for the content of 18S rRNA (n = 3).
