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Human pancreatic islet cells |
Polylactic acid functionalized with growth factor-enriched platelet gel |
3D printed construct |
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Adequate and prompt vascularization of the graft
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Vascularization enhancement by functionalized scaffold’s ability to dispense proangiogenic factors, such as VEGF, also known to increase islet viability and function
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Avoiding surgical retrieval due to the transcutaneous refillability of the device
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Significant advantage in the case of children with diabetes
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Farina et al. 2017 |
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Pancreatic cancer cells (Patu8902) and activated pancreatic fibroblast cells (PS1) |
Nanoshuttle (NS) composed of iron oxide, poly L-lysine and gold nanoparticles |
In vitro pancreatic tumor model |
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3D spheroids based on pancreatic cancer cells and activated pancreatic fibroblasts (400–600 μm in diameter) obtained by magnetic bioprinting
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Quick, easily adaptable and consistent, able to resemble the in vivo tumor microenvironment, comparatively inexpensive method
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Efficient tool for the tumor biology and drug screening studies
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Noel et al. 2017 |
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Human colorectal adenocarcinoma cell line HT-29, human pancreatic epithelial carcinoma cell line PANC-1 |
Nanoshuttle (NS) composed of iron oxide, poly L-lysine and gold nanoparticles |
Primary pancreatic organoid tumor models |
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Development of an efficient high-throughput screening (HTS) method for the production of organoids, by combining the use of a cell-repellent surface with a magnetic force-based bioprinting technology
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Validation by investigating the anticancer agents’ effects against four patient-derived pancreatic cancer KRAS mutant-associated primary cells
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Cytotoxicity pilot screen of ~3300 approved drugs
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Readily applicability to support large-scale clinical drug screening on ex vivo 3D tumor models directly harvested from patients
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Hou et al. 2018 |
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Pancreatic cancer cell lines, i.e., MIA PaCa-2 and PANC-1 |
NanoShuttle nanoparticles (Nano3D Biosciences Inc., Houston, TX, USA) |
Spheroids from MIA PaCa-2 and PANC-1 cells, mixed with human fibroblasts in a ratio of 1:1, and incubated with NanoShuttle nanoparticles |
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The greatest effect on tumor spheroid growth in both cell lines with the combinations of ICPD47, inhibitor of Hsp90 (heat shock protein 90) with the antimetabolites gemcitabine (GEM) and 5-fluorouracil (5-FU) in a ratio of 1:5
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Significant dropping of the EC50 value in PANC-1 cell line from 4.04 ± 0.046 to 1.68 ± 0.004 μM, in the case of the ICPD47 combination with mild hyperthermia
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Synergistic action of the Hsp90 inhibitors, i.e., ICPD47 and ICPD62, with GEM, 5-FU and the topoisomerase inhibitor doxorubicin (DOX), under the same conditions
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Daunys et al. 2019 |
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Human primary pancreatic stellate cells (PSCs), human umbilical vein endothelial cells (HUVECs), HMF, subcutaneous preadipocytes(SPA), and MCF-7 cells |
Alginate-containing hydrogel |
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Application of 3D bioprinting to generate multicellular, architecturally defined, scaffold-free tissue models of human tumors
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Use of Organovo’s Novogen MMX Bioprinter Platform to print structures composed of a cancer cell core surrounded by several stromal cell types
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Langer et al. 2019 |
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AR42J-B-13 rat acinar cell line |
Methacrylated gelatin (GELMA) |
Laser-assisted bioprinted 3D pancreatic cell spheroid arrays |
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Suitability of the laser-assisted bioprinting to generate cellular spheroid arrays with high control over cell number deposition and spatial resolution
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Replication of the initial stages of the pancreatic ductal adenocarcinoma by means of the bioprinted miniaturized spheroid-based array model, composed of both acinar and ductal cells
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Utility of the model to study the internal and external factors that contribute to the precursor PDAC lesions formation and to cancer progression
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Hakobyan et al. 2020 |
