FIG. 4.

In vivo interaction between MAZR and Bach2. (A) Schematic representation of GST-fused MAZR and FLAG-tagged MAZR (fMAZR). Dotted box indicates the FLAG epitope. (B and C) GST-MAZR, Bach2 and fMAZR were transiently expressed in 293T cells in various combinations as indicated. Whole-cell extracts were incubated with glutathione beads and precipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting using anti-Bach2 (αF69-2 serum) (B) and anti-FLAG (C). Positions of molecular markers (116 and 83 kDa) are shown on the left. G, GST; G-M, GST-MAZR.