Figure 1.

HOXB7 expression in MDA-MB-468 cells transfected with siHOXB7 and control siNEG evaluated by RT-qPCR (A) and western-blot (B) analyses at 24, 48, and 72 h (h) post-transfection (three replicates). Statistical analyses and significant differences are indicated with asterisks based on Test-t analyses (*, p < 0.001). Expression differences between time points were compared using the post hoc Tukey test. The HOXB7 mRNA levels were approximately 70% lower in HOXB7-silenced cells than in the controls, and the protein levels were also lower in the HOXB7-silenced cells just detectable at 24 h and 48 h post-transfection. In the western-blot analyses, β-tubulin was used as the reference protein.