Figure 4.

Influence of HOXB7 overexpression in CDH1 regulation in MDA-MB-468 triple-negative breast cancer cells. (A) Expression analyses of CDH1 in 5-Aza-2′deoxycytidine treated cells analyzed by RT-qPCR. Three independent biological replicates were used in the statistical analyses, and significant differences were indicated with asterisks based on the analyses using t-test (**, p < 0.05, *, p < 0.001). (B) Methylation status of the CDH1 promoter in HOXB7-silenced cells and controls. Bisulfite sequencing analyses revealed lower CDH1 promoter methylation in cells transfected with siHOXB7 than in controls transfected with siNEG. CpGs regions are identified by squares: black squares represent methylated cytosine and white squares represent unmethylated cytosine. Each row represents a single clone.