Coronal sections showing Fgf8 fate map at T-E7.5 (red, TDT) with immunolabeling for TH (blue) and TPH (green). (a) TDT is intermixed with the midbrain DA cell groups. (b) The retrorubral field (RRF) and A10 dorsocaudal (A10dc) groups contain TDT, although only a minority of DA neurons have detectable dual labeling. (c, d, e, f) TDT labeling extends ventral to the xscp and includes part of the MnR and B9 area. (g, h, i) TDT is found in the entire DR, including the most caudal extent medial to the DTg. Scale bar in (i) = 250 um.