Figure 7:

Bilateral infusion of a mixture of clock genes antisense oligodeoxynucleotide into the shell region of nucleus accumbens (NAcSh) did not produce any significant effect on sucrose consumption.

Panel A: A Coronal schematic [Figure 21, AP = 1.2 mm, adapted from Franklin and Paxinos, (2008)] showing target sites of the bilateral microinjector cannulas localized in the NAcSh (localized between anteroposterior levels 1.5 and 1.1) of the mice infused with antisense oligodeoxynucleotides (Antisense Group; N = 5; Red circles) and random oligodeoxynucleotides (Control Group; N = 5; black circles). ac = anterior commissure, LV = lateral ventricles, AP = antero-posterior.

Panel B: A representative photomicrograph showing lesions caused by bilateral microinfusions (black arrows) in the NAcSh. ac = anterior commissure, LV = lateral ventricles. Scale bar = 100 μm.

Panel C: A graphical representation depicting no significant change in sucrose consumption during four hours on Day 4 of drinking-in-the-dark paradigm in mice infused with a mixture of clock genes antisense oligodeoxynucleotide into the NAcSh (Antisense group; N = 5) as compared to the Controls (N = 5).