Figure 4.

AKT inhibitor modulates EGFR-induced AR expression and nuclear translocation in the U87MG glioblastoma cell line model. (A) Immunofluorescence staining of AR in U87MG subclones following treatment with AKT kinase inhibitor. Immunofluorescence staining of anti-AR (D6F11) (red) in U87MG WT EGFR or EGFRvIII following 6 h treatment with vehicle or 5 µm MK2206. DAPI staining indicates the location of the nucleus (blue). Cell nuclei were stained with DAPI (blue). White arrows indicate AR nuclear staining. (B) Relative AR nuclear localization was determined by quantification of the relative fluorescent intensity ratio between nuclear and cytosolic compartments using ImageJ software. The results are expressed as mean ± SD following normalization to the vehicle-treated subclones, p-values between the groups are indicated.