Figure 7.

The combination effect of an AR antagonist and EGFR inhibitors on glioblastoma cell lines. (A) U87MG, A172, and T98G glioblastoma cells were treated for 72 h with EGFR inhibitors (striped columns): erlotinib (Erl), erbitux (Erb), afatinib (Afa), or the AR antagonist, enzalutamide (Enz; white columns), alone or in combination (black columns). Cell density is expressed as the percentage of cells treated with vehicle (gray columns). All experiments were repeated at least three times. The results are the mean ± SD. * p < 0.05 indicates significant results of each treatment compared to vehicle-treated cells (Student’s two-tailed t-test). (B) qPCR analysis results of the HPRT-normalized relative quantification of EGFR mRNA expression in the glioblastoma cells versus expression in a commercial RNA mixture of 23 normal brains (N.B).