Table 1.
The kinases with high fold phosphorylation levels in response to overexpression of EGFR that were decreased following afatinib treatment.
| Kinase | U87MG Control Pixels Densitometry | U87MG EGFR Pixels Densitometry |
EGFR-Derived Fold Induction | U87MG EGFR + Afatinib Pixels Densitometry |
Afatinib-Derived Fold Reduction |
|---|---|---|---|---|---|
| EGFR (Y1086) | 14 | 203 | 14.5 | 1 | 203 |
| Yes (Y426) | 117 | 397 | 3.4 | 1 | 397 |
| p53 (S392) | 1406 | 2412 | 1.7 | 682 | 3.5 |
| p38 alpha (T180/Y182) | 7 | 904 | 129 | 174 | 5.2 |
| HSP27 (S78/S82) | 381 | 6336 | 17 | 1 | 6336 |
| MSK1/2 (S376/S360) | 4191 | 8184 | 1.95 | 525 | 15.6 |
| AKT 1/2/3 (S473) | 7060 | 10,753 | 1.5 | 1015 | 10.6 |
| JNK 1/2/3 (T183/Y185, T221/Y223) | 774 | 2305 | 3 | 341 | 6.8 |