Figure 4.

Possible involvement of cytoplasmic bleb in the elimination of extrachromosomal circles. Large cytoplasmic bleb (protrusion) induced by fresh serum could entrap the micronuclei [31]. The bottom of such bleb was constricted (grayscale DIC image), where actin (red in (A)) and phosphorylated myosin (MRLC-2P; green in (B)) was located (noted as “+” in (B)) just like a contractile ring during the cytokinesis. DMs were detected in green by Lactose repressor-GFP binding to lactose operator sequence on DMs [29]. These are unpublished images.