FIG. 6.

Localization of A6/twinfilin in NIH 3T3 (panels A to F) and N18 (G and H) cells. The cells were stained with FITC-conjugated DNase I (C and F) to visualize G-actin, with rhodamine-phalloidin (B, E, and H) to visualize F-actin, and with anti-A6/twinfilin antibody (A, D, and G). DNase I also shows bright nuclear staining and therefore could not be used for visualization of G-actin in N18 cells. A6/twinfilin shows strong punctate cytoplasmic staining in NIH 3T3 and N18 cells, but in NIH 3T3 cells it is also concentrated in those cortical actin structures which overlap with high concentrations of G-actin. In N18 cells, A6/twinfilin is concentrated at the F-actin-rich filopodia. Scale bar, 10 μm.