FIG. 9.

Effects of small GTPases on A6/twinfilin localization in NIH 3T3 cells. Cells were transfected with Cdc42(V12) (A to C), RhoA(V14) (D to F), active Rac1(V12) (G to I), and inactive Rac1(V12,N17) (J to L). (A, D, G, and J) Localization of the GTPases as GFP fusion proteins. Cells are stained with rhodamine-phalloidin to visualize F-actin (B, E, H, and K) and with anti-A6/twinfilin antibody (C, F, I, and L). A6/twinfilin colocalizes with the activated forms of Rac1 and Cdc42 to membrane ruffles and to cell-cell contacts, respectively. The activated form of Rac1(V12) induces A6/twinfilin localization to the nucleus and cell cortex. Scale bar, 10 μm.