Figure 5.

Physical interactions among RsMYB1^Full, RsMYB1^Short, and RsTT8. (A) Schematic diagrams of the constructs used in the Y2H assay. The amino acid positions of the fragments are shown. (B) Protein–protein interactions among RsMYB1^Full, RsMYB1^Short, and RsTT8, as revealed by Y2H analysis. SD/−LT, synthetic defined medium lacking Leu and Trp; SD/−LTH+3AT, SD medium lacking Leu, Trp, and His but containing 10 mM 3-amino-1,2,4-triazole (AT). AD and BD indicate the GAL4 activation domain and binding domain, respectively.