The antagonistic activity of melatonin and the 5-methoxyindole toward F. graminearum PH-1. (A,B) F. graminearum has grown in different media (PDA, CM and MM) containing different concentrations of melatonin (0, 0.1, 0.5, 1, 2 and 4 mM) grown for 3 days, and EC50 of melatonin were evaluated. (C,D) F. graminearum has grown in different medias containing different concentrations of 5-methoxyindole grown for 3 days, and the EC50 of 5-methoxyindole were evaluated. (E) The EC50 of melatonin and 5-methoxyindole were evaluated in the media of PDA. The experiment was repeated independently three times, and 0.05% (vol/vol) methanol/water served as the ck. The error bars represent the mean standard deviation of each treatment repeated three times with three replicates. The letters above the columns indicate significant differences. The significant difference between the treatments was determined through Tukey’s HSD test at p ≤ 0.05.