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. 2021 Oct 12;22(20):10991. doi: 10.3390/ijms222010991

Figure 5.

Figure 5

The ROS production of F. graminearum PH-1 hyphae and conidia after being treated with melatonin and 5-methoxyindole. (A,B) The ROS detection of F. graminearum hyphae and conidia were based on DCFH-DA staining after treatment with 4 mM melatonin and the 0.1 mM 5-methoxyindole grown for 3 days; (C,D) Average fluorescence analysis of F. graminearum hyphae after treatment with the melatonin and 5-methoxyindole grown for 3 days; (E,F) average fluorescence analysis of F. graminearum conidia after treatment with the melatonin and 5-methoxyindole grown for 3 days. The experiment was repeated independently three times, and 0.05% (vol/vol) methanol/water served as the ck. The error bars represent the mean standard deviation of each treatment repeated three times with three replicates. The letters above the columns indicate significant difference. The significant difference between the treatments was determined through Tukey’s HSD test at p ≤ 0.05. The higher level of green fluorescence indicates the high level of ROS induction in F. graminearum exposed to melatonin and 5-methoxyindole.