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. 2021 Oct 2;12(10):1574. doi: 10.3390/genes12101574

Figure 13.

Figure 13

Post-thaw (PT) percentage of viable (YO-PRO-1−/PI−), plasma membrane apoptotic (YO-PRO-1+/PI−), and dead spermatozoa (YO-PRO-1+/PI+) following storage in Beltsville Thawing Solution (BTS) and fractionated seminal plasma (SP1 and SP2) of boar ejaculates at different periods. (A) 0 h and (B) 2h PT storage for YO-PRO-1−/PI− stained spermatozoa, (C) 0 h and (D) 2 h PT storage for YO-PRO-1+/PI− stained spermatozoa, and (E) 0 h and (F) 2 h PT storage for YO-PRO-1+/PI+ stained spermatozoa. Values are expressed as the means (± SEM) of four to five ejaculates from seven boars. Values with different letters (a,b) within treatment are significant at p < 0.05.