FIG. 2.

Loss of functional mtDNA in Δhmi1 strains. (A) Southern blot of total cellular DNA. DNA, isolated from the wt strain W303-1A (wt) and from the haploid Δhmi1 colonies (Δ), was cut with EcoRI (lanes 1, 2, 5, and 6) or BamHI (lanes 3, 4, 7, and 8) and probed with mitochondrial COX2 (lanes 1 to 4) or nuclear DNA2 (lanes 5 to 8) probes. (B) DNA staining in nuclei and mitochondria of the wt W303-1A strain (panel 1) and of a haploid cell population from a colony where the HMI1 ORF has been disrupted (panel 2).