FIG. 3.
The recombinant Hmi1 protein is a DNA helicase. (A) Purification of the Hmi1 protein. Lane 1, glutathione agarose eluate; lane 2, thrombin-cleaved material; lane 3, 400 mM S-Sepharose fraction; lane 4, protein size markers BSA (68 kDa), ovalbumin (45 kDa), and trypsinogen (24 kDa). (B) DNA unwinding assay with the recombinant Hmi1 protein. Lane 1, assay with 0.2 μg of Hmi1p; lanes 2 to 4, assays with 0.4 μg of Hmi1; lane 3, omitted ATP; lane 4, omitted MgCl2; lane 5, substrate DNA; lane 6, heat-denatured substrate.