Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Mar;20(5):1816–1824. doi: 10.1128/mcb.20.5.1816-1824.2000

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2000, American Society for Microbiology

PMC Copyright notice

FIG. 3 — The recombinant Hmi1 protein is a DNA helicase. (A) Purification of the Hmi1 protein. Lane 1, glutathione agarose eluate; lane 2, thrombin-cleaved material; lane 3, 400 mM S-Sepharose fraction; lane 4, protein size markers BSA (68 kDa), ovalbumin (45 kDa), and trypsinogen (24 kDa). (B) DNA unwinding assay with the recombinant Hmi1 protein. Lane 1, assay with 0.2 μg of Hmi1p; lanes 2 to 4, assays with 0.4 μg of Hmi1; lane 3, omitted ATP; lane 4, omitted MgCl₂; lane 5, substrate DNA; lane 6, heat-denatured substrate.