FIG. 4.

Active transcription in mitochondria of Δhmi1 strains. Total cellular RNAs from [rho⁰] strain SK088 and [rho⁻] hypersuppressive strains SK061 and SK041 were analyzed by dot blot hybridization. The amount of total RNA loaded per dot is indicated. Lanes marked “+KOH” represent alkali-treated samples. Lanes marked “+HMI1” are samples from strains that were transformed with the HMI1 complementing plasmid pRS315-HMI1. The blot was probed with a mitochondrial ori2 probe (left panel) and then stripped and probed with a cytoplasmic 25S rRNA probe (right panel).